Summary
Type: SPE
Application #: 301950
Catalog#: 730013 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Column conditioning:
1 column volume methanol, then 1 column volume water
Sample application:
Slowly force or aspirate the diluted sample through the column
Column washing:
3 x 3 ml water, then 2 x 3 ml methanol, finally 2 x 3 ml ethyl acetate
Elution:
4 ml methanol / ethyl acetate (1 : 1, v/v) containing 0.01 % ammonium chloride;
concentrate eluate in a stream of nitrogen
Authors: Macherey-Nagel
Source: LC department